spin column Search Results


rna  (New England Biolabs)
99
New England Biolabs rna
(A) Schematic overview of the tRNA silica-based spin column purification process. First, the total <t>RNA</t> sample is mixed with long RNA binding buffer (LBB) and bound to the first column. The flowthrough will contain RNAs < 120 bp. This flowthrough is mixed with short RNA binding buffer (SBB) and bound to the second column. The RNA bound to the column is then washed with a chaotropic wash (CW), ethanol wash (EW) and eluted with ultra-pure water. (B–D) RNA samples analysed on 8–10% denaturing urea-PAA gels. Marker: low-range ssRNA (NEB). Bands corresponding to 5.8S and 5S rRNA are indicated. (B) Binding capacity of Monarch RNA Cleanup Columns (10 μg, NEB) loaded with 10, 5, or 2.5 µg total RNA. 50 ng RNA loaded to gel from column I and II eluates. Input – total RNA post-phenol/BCP extraction; DA/DPH <t>–</t> <t>deacylated</t> and dephosphorylated total RNA; CTRL – only dephosphorylated total RNA; I/II – eluates from column I and II. (C) Equal volumes loaded on gel from column I and II eluates. (D) Column and gel-extracted tRNA used in the tRNA-seq library preparation. (E) PCA clustering of sequenced samples (n=48). Green circle: column extracted tRNA; black circle: gel-extracted tRNA. (F) Correlation of normalized peak heights of global tRNA modifications detected by LC-MS between extraction methods (log 2 scale; column extraction n=5, gel extraction n=3). Pearson’s r = 0.9998, R² = 0.9995.
Rna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chroma spin 1 te 100 columns  (TaKaRa)
94
TaKaRa chroma spin 1 te 100 columns
(A) Schematic overview of the tRNA silica-based spin column purification process. First, the total <t>RNA</t> sample is mixed with long RNA binding buffer (LBB) and bound to the first column. The flowthrough will contain RNAs < 120 bp. This flowthrough is mixed with short RNA binding buffer (SBB) and bound to the second column. The RNA bound to the column is then washed with a chaotropic wash (CW), ethanol wash (EW) and eluted with ultra-pure water. (B–D) RNA samples analysed on 8–10% denaturing urea-PAA gels. Marker: low-range ssRNA (NEB). Bands corresponding to 5.8S and 5S rRNA are indicated. (B) Binding capacity of Monarch RNA Cleanup Columns (10 μg, NEB) loaded with 10, 5, or 2.5 µg total RNA. 50 ng RNA loaded to gel from column I and II eluates. Input – total RNA post-phenol/BCP extraction; DA/DPH <t>–</t> <t>deacylated</t> and dephosphorylated total RNA; CTRL – only dephosphorylated total RNA; I/II – eluates from column I and II. (C) Equal volumes loaded on gel from column I and II eluates. (D) Column and gel-extracted tRNA used in the tRNA-seq library preparation. (E) PCA clustering of sequenced samples (n=48). Green circle: column extracted tRNA; black circle: gel-extracted tRNA. (F) Correlation of normalized peak heights of global tRNA modifications detected by LC-MS between extraction methods (log 2 scale; column extraction n=5, gel extraction n=3). Pearson’s r = 0.9998, R² = 0.9995.
Chroma Spin 1 Te 100 Columns, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nebexpress ni spin columns  (New England Biolabs)
95
New England Biolabs nebexpress ni spin columns
(A) Schematic overview of the tRNA silica-based spin column purification process. First, the total <t>RNA</t> sample is mixed with long RNA binding buffer (LBB) and bound to the first column. The flowthrough will contain RNAs < 120 bp. This flowthrough is mixed with short RNA binding buffer (SBB) and bound to the second column. The RNA bound to the column is then washed with a chaotropic wash (CW), ethanol wash (EW) and eluted with ultra-pure water. (B–D) RNA samples analysed on 8–10% denaturing urea-PAA gels. Marker: low-range ssRNA (NEB). Bands corresponding to 5.8S and 5S rRNA are indicated. (B) Binding capacity of Monarch RNA Cleanup Columns (10 μg, NEB) loaded with 10, 5, or 2.5 µg total RNA. 50 ng RNA loaded to gel from column I and II eluates. Input – total RNA post-phenol/BCP extraction; DA/DPH <t>–</t> <t>deacylated</t> and dephosphorylated total RNA; CTRL – only dephosphorylated total RNA; I/II – eluates from column I and II. (C) Equal volumes loaded on gel from column I and II eluates. (D) Column and gel-extracted tRNA used in the tRNA-seq library preparation. (E) PCA clustering of sequenced samples (n=48). Green circle: column extracted tRNA; black circle: gel-extracted tRNA. (F) Correlation of normalized peak heights of global tRNA modifications detected by LC-MS between extraction methods (log 2 scale; column extraction n=5, gel extraction n=3). Pearson’s r = 0.9998, R² = 0.9995.
Nebexpress Ni Spin Columns, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bio rad p6 micro  (Bio-Rad)
96
Bio-Rad bio rad p6 micro
(A) Schematic overview of the tRNA silica-based spin column purification process. First, the total <t>RNA</t> sample is mixed with long RNA binding buffer (LBB) and bound to the first column. The flowthrough will contain RNAs < 120 bp. This flowthrough is mixed with short RNA binding buffer (SBB) and bound to the second column. The RNA bound to the column is then washed with a chaotropic wash (CW), ethanol wash (EW) and eluted with ultra-pure water. (B–D) RNA samples analysed on 8–10% denaturing urea-PAA gels. Marker: low-range ssRNA (NEB). Bands corresponding to 5.8S and 5S rRNA are indicated. (B) Binding capacity of Monarch RNA Cleanup Columns (10 μg, NEB) loaded with 10, 5, or 2.5 µg total RNA. 50 ng RNA loaded to gel from column I and II eluates. Input – total RNA post-phenol/BCP extraction; DA/DPH <t>–</t> <t>deacylated</t> and dephosphorylated total RNA; CTRL – only dephosphorylated total RNA; I/II – eluates from column I and II. (C) Equal volumes loaded on gel from column I and II eluates. (D) Column and gel-extracted tRNA used in the tRNA-seq library preparation. (E) PCA clustering of sequenced samples (n=48). Green circle: column extracted tRNA; black circle: gel-extracted tRNA. (F) Correlation of normalized peak heights of global tRNA modifications detected by LC-MS between extraction methods (log 2 scale; column extraction n=5, gel extraction n=3). Pearson’s r = 0.9998, R² = 0.9995.
Bio Rad P6 Micro, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bio spin 30 columns  (Bio-Rad)
95
Bio-Rad bio spin 30 columns
(A) Schematic overview of the tRNA silica-based spin column purification process. First, the total <t>RNA</t> sample is mixed with long RNA binding buffer (LBB) and bound to the first column. The flowthrough will contain RNAs < 120 bp. This flowthrough is mixed with short RNA binding buffer (SBB) and bound to the second column. The RNA bound to the column is then washed with a chaotropic wash (CW), ethanol wash (EW) and eluted with ultra-pure water. (B–D) RNA samples analysed on 8–10% denaturing urea-PAA gels. Marker: low-range ssRNA (NEB). Bands corresponding to 5.8S and 5S rRNA are indicated. (B) Binding capacity of Monarch RNA Cleanup Columns (10 μg, NEB) loaded with 10, 5, or 2.5 µg total RNA. 50 ng RNA loaded to gel from column I and II eluates. Input – total RNA post-phenol/BCP extraction; DA/DPH <t>–</t> <t>deacylated</t> and dephosphorylated total RNA; CTRL – only dephosphorylated total RNA; I/II – eluates from column I and II. (C) Equal volumes loaded on gel from column I and II eluates. (D) Column and gel-extracted tRNA used in the tRNA-seq library preparation. (E) PCA clustering of sequenced samples (n=48). Green circle: column extracted tRNA; black circle: gel-extracted tRNA. (F) Correlation of normalized peak heights of global tRNA modifications detected by LC-MS between extraction methods (log 2 scale; column extraction n=5, gel extraction n=3). Pearson’s r = 0.9998, R² = 0.9995.
Bio Spin 30 Columns, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bio spin column  (Bio-Rad)
96
Bio-Rad bio spin column
(A) Schematic overview of the tRNA silica-based spin column purification process. First, the total <t>RNA</t> sample is mixed with long RNA binding buffer (LBB) and bound to the first column. The flowthrough will contain RNAs < 120 bp. This flowthrough is mixed with short RNA binding buffer (SBB) and bound to the second column. The RNA bound to the column is then washed with a chaotropic wash (CW), ethanol wash (EW) and eluted with ultra-pure water. (B–D) RNA samples analysed on 8–10% denaturing urea-PAA gels. Marker: low-range ssRNA (NEB). Bands corresponding to 5.8S and 5S rRNA are indicated. (B) Binding capacity of Monarch RNA Cleanup Columns (10 μg, NEB) loaded with 10, 5, or 2.5 µg total RNA. 50 ng RNA loaded to gel from column I and II eluates. Input – total RNA post-phenol/BCP extraction; DA/DPH <t>–</t> <t>deacylated</t> and dephosphorylated total RNA; CTRL – only dephosphorylated total RNA; I/II – eluates from column I and II. (C) Equal volumes loaded on gel from column I and II eluates. (D) Column and gel-extracted tRNA used in the tRNA-seq library preparation. (E) PCA clustering of sequenced samples (n=48). Green circle: column extracted tRNA; black circle: gel-extracted tRNA. (F) Correlation of normalized peak heights of global tRNA modifications detected by LC-MS between extraction methods (log 2 scale; column extraction n=5, gel extraction n=3). Pearson’s r = 0.9998, R² = 0.9995.
Bio Spin Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bio spin column - by Bioz Stars, 2026-04
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bio  (Bio-Rad)
96
Bio-Rad bio
(A) Schematic overview of the tRNA silica-based spin column purification process. First, the total <t>RNA</t> sample is mixed with long RNA binding buffer (LBB) and bound to the first column. The flowthrough will contain RNAs < 120 bp. This flowthrough is mixed with short RNA binding buffer (SBB) and bound to the second column. The RNA bound to the column is then washed with a chaotropic wash (CW), ethanol wash (EW) and eluted with ultra-pure water. (B–D) RNA samples analysed on 8–10% denaturing urea-PAA gels. Marker: low-range ssRNA (NEB). Bands corresponding to 5.8S and 5S rRNA are indicated. (B) Binding capacity of Monarch RNA Cleanup Columns (10 μg, NEB) loaded with 10, 5, or 2.5 µg total RNA. 50 ng RNA loaded to gel from column I and II eluates. Input – total RNA post-phenol/BCP extraction; DA/DPH <t>–</t> <t>deacylated</t> and dephosphorylated total RNA; CTRL – only dephosphorylated total RNA; I/II – eluates from column I and II. (C) Equal volumes loaded on gel from column I and II eluates. (D) Column and gel-extracted tRNA used in the tRNA-seq library preparation. (E) PCA clustering of sequenced samples (n=48). Green circle: column extracted tRNA; black circle: gel-extracted tRNA. (F) Correlation of normalized peak heights of global tRNA modifications detected by LC-MS between extraction methods (log 2 scale; column extraction n=5, gel extraction n=3). Pearson’s r = 0.9998, R² = 0.9995.
Bio, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
bio - by Bioz Stars, 2026-04
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spin 6 column  (Bio-Rad)
96
Bio-Rad spin 6 column
(A) Schematic overview of the tRNA silica-based spin column purification process. First, the total <t>RNA</t> sample is mixed with long RNA binding buffer (LBB) and bound to the first column. The flowthrough will contain RNAs < 120 bp. This flowthrough is mixed with short RNA binding buffer (SBB) and bound to the second column. The RNA bound to the column is then washed with a chaotropic wash (CW), ethanol wash (EW) and eluted with ultra-pure water. (B–D) RNA samples analysed on 8–10% denaturing urea-PAA gels. Marker: low-range ssRNA (NEB). Bands corresponding to 5.8S and 5S rRNA are indicated. (B) Binding capacity of Monarch RNA Cleanup Columns (10 μg, NEB) loaded with 10, 5, or 2.5 µg total RNA. 50 ng RNA loaded to gel from column I and II eluates. Input – total RNA post-phenol/BCP extraction; DA/DPH <t>–</t> <t>deacylated</t> and dephosphorylated total RNA; CTRL – only dephosphorylated total RNA; I/II – eluates from column I and II. (C) Equal volumes loaded on gel from column I and II eluates. (D) Column and gel-extracted tRNA used in the tRNA-seq library preparation. (E) PCA clustering of sequenced samples (n=48). Green circle: column extracted tRNA; black circle: gel-extracted tRNA. (F) Correlation of normalized peak heights of global tRNA modifications detected by LC-MS between extraction methods (log 2 scale; column extraction n=5, gel extraction n=3). Pearson’s r = 0.9998, R² = 0.9995.
Spin 6 Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gel filtration spin columns  (Bio-Rad)
95
Bio-Rad gel filtration spin columns
(A) Schematic overview of the tRNA silica-based spin column purification process. First, the total <t>RNA</t> sample is mixed with long RNA binding buffer (LBB) and bound to the first column. The flowthrough will contain RNAs < 120 bp. This flowthrough is mixed with short RNA binding buffer (SBB) and bound to the second column. The RNA bound to the column is then washed with a chaotropic wash (CW), ethanol wash (EW) and eluted with ultra-pure water. (B–D) RNA samples analysed on 8–10% denaturing urea-PAA gels. Marker: low-range ssRNA (NEB). Bands corresponding to 5.8S and 5S rRNA are indicated. (B) Binding capacity of Monarch RNA Cleanup Columns (10 μg, NEB) loaded with 10, 5, or 2.5 µg total RNA. 50 ng RNA loaded to gel from column I and II eluates. Input – total RNA post-phenol/BCP extraction; DA/DPH <t>–</t> <t>deacylated</t> and dephosphorylated total RNA; CTRL – only dephosphorylated total RNA; I/II – eluates from column I and II. (C) Equal volumes loaded on gel from column I and II eluates. (D) Column and gel-extracted tRNA used in the tRNA-seq library preparation. (E) PCA clustering of sequenced samples (n=48). Green circle: column extracted tRNA; black circle: gel-extracted tRNA. (F) Correlation of normalized peak heights of global tRNA modifications detected by LC-MS between extraction methods (log 2 scale; column extraction n=5, gel extraction n=3). Pearson’s r = 0.9998, R² = 0.9995.
Gel Filtration Spin Columns, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dna purification buffers  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc dna purification buffers
(A) Schematic overview of the tRNA silica-based spin column purification process. First, the total <t>RNA</t> sample is mixed with long RNA binding buffer (LBB) and bound to the first column. The flowthrough will contain RNAs < 120 bp. This flowthrough is mixed with short RNA binding buffer (SBB) and bound to the second column. The RNA bound to the column is then washed with a chaotropic wash (CW), ethanol wash (EW) and eluted with ultra-pure water. (B–D) RNA samples analysed on 8–10% denaturing urea-PAA gels. Marker: low-range ssRNA (NEB). Bands corresponding to 5.8S and 5S rRNA are indicated. (B) Binding capacity of Monarch RNA Cleanup Columns (10 μg, NEB) loaded with 10, 5, or 2.5 µg total RNA. 50 ng RNA loaded to gel from column I and II eluates. Input – total RNA post-phenol/BCP extraction; DA/DPH <t>–</t> <t>deacylated</t> and dephosphorylated total RNA; CTRL – only dephosphorylated total RNA; I/II – eluates from column I and II. (C) Equal volumes loaded on gel from column I and II eluates. (D) Column and gel-extracted tRNA used in the tRNA-seq library preparation. (E) PCA clustering of sequenced samples (n=48). Green circle: column extracted tRNA; black circle: gel-extracted tRNA. (F) Correlation of normalized peak heights of global tRNA modifications detected by LC-MS between extraction methods (log 2 scale; column extraction n=5, gel extraction n=3). Pearson’s r = 0.9998, R² = 0.9995.
Dna Purification Buffers, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dna purification buffers - by Bioz Stars, 2026-04
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zymo spin ic columns  (Zymo Research)
96
Zymo Research zymo spin ic columns
(A) Schematic overview of the tRNA silica-based spin column purification process. First, the total <t>RNA</t> sample is mixed with long RNA binding buffer (LBB) and bound to the first column. The flowthrough will contain RNAs < 120 bp. This flowthrough is mixed with short RNA binding buffer (SBB) and bound to the second column. The RNA bound to the column is then washed with a chaotropic wash (CW), ethanol wash (EW) and eluted with ultra-pure water. (B–D) RNA samples analysed on 8–10% denaturing urea-PAA gels. Marker: low-range ssRNA (NEB). Bands corresponding to 5.8S and 5S rRNA are indicated. (B) Binding capacity of Monarch RNA Cleanup Columns (10 μg, NEB) loaded with 10, 5, or 2.5 µg total RNA. 50 ng RNA loaded to gel from column I and II eluates. Input – total RNA post-phenol/BCP extraction; DA/DPH <t>–</t> <t>deacylated</t> and dephosphorylated total RNA; CTRL – only dephosphorylated total RNA; I/II – eluates from column I and II. (C) Equal volumes loaded on gel from column I and II eluates. (D) Column and gel-extracted tRNA used in the tRNA-seq library preparation. (E) PCA clustering of sequenced samples (n=48). Green circle: column extracted tRNA; black circle: gel-extracted tRNA. (F) Correlation of normalized peak heights of global tRNA modifications detected by LC-MS between extraction methods (log 2 scale; column extraction n=5, gel extraction n=3). Pearson’s r = 0.9998, R² = 0.9995.
Zymo Spin Ic Columns, supplied by Zymo Research, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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micro bio spin chromatography column  (Bio-Rad)
99
Bio-Rad micro bio spin chromatography column
(A) Schematic overview of the tRNA silica-based spin column purification process. First, the total <t>RNA</t> sample is mixed with long RNA binding buffer (LBB) and bound to the first column. The flowthrough will contain RNAs < 120 bp. This flowthrough is mixed with short RNA binding buffer (SBB) and bound to the second column. The RNA bound to the column is then washed with a chaotropic wash (CW), ethanol wash (EW) and eluted with ultra-pure water. (B–D) RNA samples analysed on 8–10% denaturing urea-PAA gels. Marker: low-range ssRNA (NEB). Bands corresponding to 5.8S and 5S rRNA are indicated. (B) Binding capacity of Monarch RNA Cleanup Columns (10 μg, NEB) loaded with 10, 5, or 2.5 µg total RNA. 50 ng RNA loaded to gel from column I and II eluates. Input – total RNA post-phenol/BCP extraction; DA/DPH <t>–</t> <t>deacylated</t> and dephosphorylated total RNA; CTRL – only dephosphorylated total RNA; I/II – eluates from column I and II. (C) Equal volumes loaded on gel from column I and II eluates. (D) Column and gel-extracted tRNA used in the tRNA-seq library preparation. (E) PCA clustering of sequenced samples (n=48). Green circle: column extracted tRNA; black circle: gel-extracted tRNA. (F) Correlation of normalized peak heights of global tRNA modifications detected by LC-MS between extraction methods (log 2 scale; column extraction n=5, gel extraction n=3). Pearson’s r = 0.9998, R² = 0.9995.
Micro Bio Spin Chromatography Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) Schematic overview of the tRNA silica-based spin column purification process. First, the total RNA sample is mixed with long RNA binding buffer (LBB) and bound to the first column. The flowthrough will contain RNAs < 120 bp. This flowthrough is mixed with short RNA binding buffer (SBB) and bound to the second column. The RNA bound to the column is then washed with a chaotropic wash (CW), ethanol wash (EW) and eluted with ultra-pure water. (B–D) RNA samples analysed on 8–10% denaturing urea-PAA gels. Marker: low-range ssRNA (NEB). Bands corresponding to 5.8S and 5S rRNA are indicated. (B) Binding capacity of Monarch RNA Cleanup Columns (10 μg, NEB) loaded with 10, 5, or 2.5 µg total RNA. 50 ng RNA loaded to gel from column I and II eluates. Input – total RNA post-phenol/BCP extraction; DA/DPH – deacylated and dephosphorylated total RNA; CTRL – only dephosphorylated total RNA; I/II – eluates from column I and II. (C) Equal volumes loaded on gel from column I and II eluates. (D) Column and gel-extracted tRNA used in the tRNA-seq library preparation. (E) PCA clustering of sequenced samples (n=48). Green circle: column extracted tRNA; black circle: gel-extracted tRNA. (F) Correlation of normalized peak heights of global tRNA modifications detected by LC-MS between extraction methods (log 2 scale; column extraction n=5, gel extraction n=3). Pearson’s r = 0.9998, R² = 0.9995.

Journal: bioRxiv

Article Title: In-house produced MarathonRT comparison to uMRT and Induro in tRNA sequencing library preparation

doi: 10.64898/2026.03.09.710538

Figure Lengend Snippet: (A) Schematic overview of the tRNA silica-based spin column purification process. First, the total RNA sample is mixed with long RNA binding buffer (LBB) and bound to the first column. The flowthrough will contain RNAs < 120 bp. This flowthrough is mixed with short RNA binding buffer (SBB) and bound to the second column. The RNA bound to the column is then washed with a chaotropic wash (CW), ethanol wash (EW) and eluted with ultra-pure water. (B–D) RNA samples analysed on 8–10% denaturing urea-PAA gels. Marker: low-range ssRNA (NEB). Bands corresponding to 5.8S and 5S rRNA are indicated. (B) Binding capacity of Monarch RNA Cleanup Columns (10 μg, NEB) loaded with 10, 5, or 2.5 µg total RNA. 50 ng RNA loaded to gel from column I and II eluates. Input – total RNA post-phenol/BCP extraction; DA/DPH – deacylated and dephosphorylated total RNA; CTRL – only dephosphorylated total RNA; I/II – eluates from column I and II. (C) Equal volumes loaded on gel from column I and II eluates. (D) Column and gel-extracted tRNA used in the tRNA-seq library preparation. (E) PCA clustering of sequenced samples (n=48). Green circle: column extracted tRNA; black circle: gel-extracted tRNA. (F) Correlation of normalized peak heights of global tRNA modifications detected by LC-MS between extraction methods (log 2 scale; column extraction n=5, gel extraction n=3). Pearson’s r = 0.9998, R² = 0.9995.

Article Snippet: The deacylated and dephosphorylated total RNA was purified on NEB Monarch RNA Cleanup Columns (10μg) (T2037) or Macherey-Nagel NucleoSpin RNA XS Columns (740902.50S) following the published protocol , 16,000 g was used for all centrifugation steps as recommended by the column’s manufacturer.

Techniques: Purification, RNA Binding Assay, Marker, Binding Assay, Extraction, Liquid Chromatography with Mass Spectroscopy, Gel Extraction